9VFZ

Crystal structure of Phaeodactylibacter sp. phosphoglucomutase in complex with glucose-1-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97861
Spacegroup name	C 1 2 1
Unit cell lengths	108.970, 58.966, 99.517
Unit cell angles	90.00, 118.22, 90.00

Refinement procedure

Resolution	49.160 - 1.950
R-factor	0.2769
Rwork	0.272
R-free	0.35860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.432
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.950	1.950
Number of reflections	40136	2967
<I/σ(I)>	8.14
Completeness [%]	99.3	99.7
Redundancy	6.48
CC(1/2)	0.935	0.694

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.15 M DL-maleic acid pH 7.0 0.1 M Imidazole pH 7.0 24% v/v polyethylene glycol monomethyl ether 550 3 mM G1P