9VFD

Cytochrome P450 CYP153A99 from Pseudomonas sp. 19-rlim, complexed with dehydroabietic acid

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2025-03-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 65
Unit cell lengths	97.709, 97.709, 101.171
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.710 - 2.100
R-factor	0.1916
Rwork	0.190
R-free	0.21210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.206
Data reduction software	iMOSFLM
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.710	2.160
High resolution limit [Å]	2.100	2.100
Rpim	0.075	0.940
Number of reflections	32014	2634
<I/σ(I)>	12.8
Completeness [%]	99.9
Redundancy	16.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	4M Sodium formate