9VFC

Crystal structure of eukaryotic heliorhodopsin Cv06 from Chromera velia strain CCMP2878

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-05
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.87313
Spacegroup name	P 1
Unit cell lengths	77.892, 77.893, 135.987
Unit cell angles	105.67, 101.16, 90.02

Refinement procedure

Resolution	42.980 - 2.600
R-factor	0.2314
Rwork	0.230
R-free	0.27130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6is6
RMSD bond length	0.002
RMSD bond angle	0.412
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	MoRDa
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.980	42.980	2.630
High resolution limit [Å]	2.500	7.210	2.500
Rpim	0.121	0.029	1.144
Number of reflections	82180	4108	4109
<I/σ(I)>	4.9	15.1	0.7
Completeness [%]	91.8	95	69
Redundancy	2.9	2.8	3.2
CC(1/2)	0.990	0.997	0.168

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	8.2	293	1.2 M sodium potassium phosphate buffer, cryoprotection in 10% (v/v) glycerol and 2.16 M sodium potassium phosphate buffer