9VEY

Ethanol dehydrogenase of Pseudomonas syringae pv. actinidiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 32
Unit cell lengths	184.280, 184.280, 77.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.630 - 2.290
R-factor	0.2045
Rwork	0.204
R-free	0.25870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.891
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.410	2.350
High resolution limit [Å]	2.290	2.290
Number of reflections	131522	262447
<I/σ(I)>	5.3
Completeness [%]	99.6
Redundancy	10.3
CC(1/2)	0.972	0.521

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	291.15	0.2 M Lithium sulfate, Tris pH 8.5, PEG 3350