9VEH

The MIDN Catch-IRF4 (A8M) fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2025-04-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 65 2 2
Unit cell lengths	87.784, 87.784, 73.536
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.340 - 2.000
R-factor	0.2477
Rwork	0.243
R-free	0.28730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.017
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.730	2.110
High resolution limit [Å]	1.990	1.990
Number of reflections	11823	1125
<I/σ(I)>	40
Completeness [%]	98.1
Redundancy	21.4
CC(1/2)	1.000	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M ammonium tartrate (pH 7.0) and 12% (w/v) PEG 3,350