9VEA

The MIDN Catch-IRF4 (A8I) fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2025-06-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 65 2 2
Unit cell lengths	88.255, 88.255, 69.794
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.740 - 3.600
R-factor	0.239
Rwork	0.237
R-free	0.27180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.645
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.740	3.730
High resolution limit [Å]	3.600	3.600
Number of reflections	2091	197
<I/σ(I)>	8.7
Completeness [%]	99.1
Redundancy	8.1
CC(1/2)	0.960	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M ammonium tartrate (pH 7.0) and 12% (w/v) PEG 3,350