9VE8

The MIDN Catch-IRF4 (A8L) fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2025-06-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 65 2 2
Unit cell lengths	88.781, 88.781, 70.833
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.050 - 2.850
R-factor	0.2345
Rwork	0.232
R-free	0.27360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.125
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.050	2.950
High resolution limit [Å]	2.850	2.850
Number of reflections	4135	388
<I/σ(I)>	15.6
Completeness [%]	99.2
Redundancy	16.9
CC(1/2)	0.970	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	4% (v/v) tacsimate (pH 4.0) and 12% (w/v) PEG 3,350