9VE7

The MIDN Catch-IRF4 (A8V) fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2025-06-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 65 2 2
Unit cell lengths	87.649, 87.649, 74.102
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.940 - 2.150
R-factor	0.2394
Rwork	0.239
R-free	0.25370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.972
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.300	2.270
High resolution limit [Å]	2.150	2.150
Number of reflections	9613	907
<I/σ(I)>	40.3
Completeness [%]	100.0
Redundancy	31.4
CC(1/2)	0.990	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M sodium malonate (pH 4.0) and 12% (w/v) PEG 3,350