9VE6

The MIDN Catch-IRF4 (F6Y) fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2024-11-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 65 2 2
Unit cell lengths	88.597, 88.597, 72.516
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.300 - 2.800
R-factor	0.2516
Rwork	0.247
R-free	0.29570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.059
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.300	2.900
High resolution limit [Å]	2.790	2.790
Number of reflections	4490	437
<I/σ(I)>	10.5
Completeness [%]	99.8
Redundancy	11.5
CC(1/2)	0.990	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	6% (v/v) tacsimate (pH 4.0) and 12% (w/v) polyethylene glycol (PEG) 3,350