9VE5

The MIDN Catch-IRF4 (V2Y) fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2025-06-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 65 2 2
Unit cell lengths	88.267, 88.267, 72.338
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.130 - 3.200
R-factor	0.2181
Rwork	0.215
R-free	0.27580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.081
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.130	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	2969	287
<I/σ(I)>	7.3
Completeness [%]	99.1
Redundancy	4.8
CC(1/2)	0.960	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.15 M cesium chloride and 15% (w/v) PEG 3,350