9VE4

The MIDN Catch-IRF4 fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	193
Detector technology	PIXEL
Collection date	2024-12-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 65 2 2
Unit cell lengths	88.640, 88.640, 72.270
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.310 - 2.550
R-factor	0.2323
Rwork	0.230
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.131
Data reduction software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.310	2.620
High resolution limit [Å]	2.550	2.550
Number of reflections	5717	566
<I/σ(I)>	29.9
Completeness [%]	97.8
Redundancy	35.8
CC(1/2)	1.000	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	6% (v/v) tacsimate (pH 4.0) and 12% (w/v) polyethylene glycol (PEG) 3,350