9VE0

hA5-6 (W30B/W53) Fab bound to SFTSV glycoprotein Gn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	66.410, 91.910, 307.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.460 - 2.680
R-factor	0.2343
Rwork	0.231
R-free	0.28840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.159
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.460	2.810
High resolution limit [Å]	2.680	2.680
Rmerge	0.061	0.617
Number of reflections	26768	3533
<I/σ(I)>	12.9
Completeness [%]	99.3
Redundancy	5.1
CC(1/2)	0.998	0.817

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION, RECRYSTALLIZATION		289	0.16 M Magnesium acetate, 0.08 M Sodium cacodylate pH 6.5, 16 %(w/v) PEG 8000, 20 %(v/v) Glycero