9VDZ

hA5-6(W30B/E45) Fab bound to SFTSV glycoprotein Gn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9791
Spacegroup name	P 2 21 21
Unit cell lengths	65.200, 91.360, 309.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.880 - 2.430
R-factor	0.2043
Rwork	0.202
R-free	0.25550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.085
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.880	2.480
High resolution limit [Å]	2.430	2.430
Rmerge	0.106	0.565
Number of reflections	70458	4266
<I/σ(I)>	9.9
Completeness [%]	99.4
Redundancy	5.7
CC(1/2)	0.995	0.659

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION, RECRYSTALLIZATION		289	8% v/v TacsimateTM pH 6.0, 20% w/v Polyethylene glycol 3,350