9VDY

hA5-6 Fab bound to SFTSV glycoprotein Gn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 2 21 21
Unit cell lengths	64.960, 91.110, 308.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.930 - 2.280
R-factor	0.2059
Rwork	0.204
R-free	0.24010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.328
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.170	2.340
High resolution limit [Å]	2.280	2.280
Rmerge	0.084	1.442
Number of reflections	84678	6157
<I/σ(I)>	18.5
Completeness [%]	100.0
Redundancy	12.7
CC(1/2)	0.999	0.719

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION, RECRYSTALLIZATION		289	0.2 M Sodium citrate tribasic dihydrate, 20% w/v Polyethylene glycol 3,350