9VDS
Crystal structure of voltage-gated sodium channel NavAb N49K/L176W mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 126.880, 126.880, 204.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.910 - 3.700 |
| R-factor | 0.3135 |
| Rwork | 0.313 |
| R-free | 0.33890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.483 |
| Data reduction software | XDS |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.800 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rpim | 0.432 | |
| Number of reflections | 9266 | 869 |
| <I/σ(I)> | 0.01715 | 4.83 |
| Completeness [%] | 99.3 | |
| Redundancy | 54.4 | |
| CC(1/2) | 1.000 | 0.986 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 9%-11% PEG MME 2000, 100mM sodium chloride, 100mM magnesium nitrate, 25mM cadmium nitrate, 100mM Tris-HCl, pH 8.4 |
