9VD8

Structure of a truncated loopA mutant from the human gut flora K. grimontii Apg in complex with glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 65 2 2
Unit cell lengths	75.870, 75.870, 401.959
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.910 - 2.390
R-factor	0.1934
Rwork	0.191
R-free	0.23650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.873
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.910	2.480
High resolution limit [Å]	2.390	2.390
Rmerge	0.150	0.992
Number of reflections	28627	2899
<I/σ(I)>	10.6
Completeness [%]	100.0
Redundancy	9.7
CC(1/2)	0.997	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289.15	0.1 M HEPES PH=7.5, 20% w/v Poly(acrylic acid sodium salt)5100