9VC7

Structural insights into the tumor suppressor ZMYND11 reveal diverse recognition mechanisms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	198
Detector technology	PIXEL
Collection date	2025-03-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97891
Spacegroup name	P 21 21 21
Unit cell lengths	70.140, 75.950, 172.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.080 - 3.350
R-factor	0.2447
Rwork	0.244
R-free	0.26240
Structure solution method	AB INITIO PHASING
RMSD bond length	0.005
RMSD bond angle	0.806
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	PHENIX (2.0_5761)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.960	3.350
High resolution limit [Å]	3.350	3.350
Number of reflections	25011	13666
<I/σ(I)>	12.8
Completeness [%]	98.8
Redundancy	19.39
CC(1/2)	0.999	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	20%PEG4000,100 Tris-HCl 8.5,200 mM Lithium Sulfate