9VBB
Crystal structure of the PDZ tandem of syntenin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 56.958, 56.958, 150.471 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.870 - 1.900 |
| R-factor | 0.2659 |
| Rwork | 0.264 |
| R-free | 0.30240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8blu |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.828 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.330 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.061 | 1.292 |
| Number of reflections | 23132 | 1412 |
| <I/σ(I)> | 20.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2 M ammonium acetate, 0.1 M BIS-TRIS, 25% PEG 3,350 |
