9VAK
Carbohydrate-binding module 32 of LnbB from Bifidobacterium bifidum, LNB complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-12-22 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.012 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 56.489, 105.802, 122.102 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.540 - 2.000 |
| R-factor | 0.1997 |
| Rwork | 0.198 |
| R-free | 0.22960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.778 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.540 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.927 | |
| Rmeas | 0.226 | |
| Rpim | 0.086 | 0.376 |
| Number of reflections | 50324 | 3663 |
| <I/σ(I)> | 7.8 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 7 |
| CC(1/2) | 0.991 | 0.721 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Lithium chloride, HEPES-NaOH, PEG 6000 |
