9V9F
Methionyl-tRNA synthetase from Staphylococcus aureus in complex with an inhibitor
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.181, 76.758, 118.751 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.964 - 2.000 |
| Rwork | 0.196 |
| R-free | 0.21850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.136 |
| Data reduction software | autoPROC |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.964 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.088 | 0.256 |
| Number of reflections | 37385 | 2747 |
| <I/σ(I)> | 22.4 | 9.9 |
| Completeness [%] | 100.0 | |
| Redundancy | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.05 M Lithium acetate, 0.1 M HEPES 7.5, 25% w/v PEG 3350 |
