9V9B

Crystal structure of the periplasmic domain of Campylobacter jejuni CadF R268E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-06
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	44.666, 76.055, 30.969
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.950 - 1.500
R-factor	0.1678
Rwork	0.167
R-free	0.17860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.776
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	17521	864
<I/σ(I)>	16.1	2
Completeness [%]	99.9	99.8
Redundancy	6.1	5.7
CC(1/2)	0.993	0.858

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 3350, ammonium sulfate