9V85

Superfolder GFP fused gp38 receptor binding domain of bacteriophage PP01

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-20
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	172.217, 172.217, 79.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.880 - 2.100
R-factor	0.1764
Rwork	0.175
R-free	0.20770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.294
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.390	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.155	2.012
Rmeas	0.158	2.051
Rpim	0.031	0.398
Total number of observations	1850456	117945
Number of reflections	70256	4444
<I/σ(I)>	20.4	2
Completeness [%]	100.0
Redundancy	26.3	26.5
CC(1/2)	0.999	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	10% (w/v) PEG8000, 0.1M Na-HEPES-MOPS, pH7.5, 3mM CaCl2, 3mM MgCl2, 20 % (v/v) ethylene glycol