9V83

Crystal Structure of Asparagine Synthetase from Entamoeba histolytica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-29
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.918
Spacegroup name	P 21 21 21
Unit cell lengths	60.252, 149.848, 170.507
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.250 - 2.380
Rwork	0.178
R-free	0.22540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.406
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.250	2.440
High resolution limit [Å]	2.100	2.100
Number of reflections	61162	4510
<I/σ(I)>	7.7
Completeness [%]	97.7
Redundancy	3
CC(1/2)	0.992	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	Ammonium sulfate, Bis tris pH 5.6, 30% PEG 3350