9V7R
The apo structure of HnH6H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97917 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.129, 43.156, 91.465 |
| Unit cell angles | 80.77, 87.93, 83.34 |
Refinement procedure
| Resolution | 19.820 - 2.450 |
| R-factor | 0.183306733218 |
| Rwork | 0.180 |
| R-free | 0.23849 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.577 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.820 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.076 | 0.344 |
| Rpim | 0.047 | 0.222 |
| Number of reflections | 23244 | 2681 |
| <I/σ(I)> | 13 | 3.5 |
| Completeness [%] | 97.7 | 97.7 |
| Redundancy | 3.5 | 3.3 |
| CC(1/2) | 0.996 | 0.851 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES (pH 7.0), 1 M ammonium sulfate and 1 M potassium chloride |
