9V72
Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-22 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 109.222, 109.222, 119.646 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.560 - 2.755 |
| R-factor | 0.2439 |
| Rwork | 0.240 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.873 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.590 | 3.040 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 14253 | 713 |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 89.1 | |
| Redundancy | 11.2 | |
| CC(1/2) | 0.995 | 0.469 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.01 M Citric/0.025 M B-T propane ph7.1, 0.018 M Sodium malonate pH 5.0, 10.9% peg3350, 0.023 M glycyl-glycyl-glycine, 0.09 M NDSB-256 |
