9V71

Structural basis of signal activation and transduction by chitin elicitor receptor kinase 1 in Oryza sativa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-22
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 61 2 2
Unit cell lengths	58.346, 58.346, 284.559
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.750 - 2.520
R-factor	0.2384
Rwork	0.234
R-free	0.27640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.598
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.750	2.650
High resolution limit [Å]	2.520	2.520
Number of reflections	9393	1203
<I/σ(I)>	6.4
Completeness [%]	89.7
Redundancy	11.6
CC(1/2)	0.985	0.446

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M Calcium acetate hydrate, 10% w/v Polyethylene glycol 3,350