9V68

Crystal structure of Phaeodactylibacter sp. Phosphoglucomutase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97861
Spacegroup name	C 1 2 1
Unit cell lengths	117.252, 59.314, 96.835
Unit cell angles	90.00, 116.31, 90.00

Refinement procedure

Resolution	49.370 - 1.580
R-factor	0.1641
Rwork	0.164
R-free	0.18360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.772
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.370	1.660
High resolution limit [Å]	1.580	1.580
Number of reflections	79073	9752
<I/σ(I)>	13
Completeness [%]	96.2
Redundancy	5.7
CC(1/2)	0.998	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.15 M DL-maleic acid pH 7.0 0.1 M Imidazole pH 6.5 20% v/v polyethylene glycol monomethyl ether 550