9V5S

Crystal structure of EF-Tu from Mycoplasma pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-21
Detector	DECTRIS PILATUS 12M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	64.382, 106.733, 257.591
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.200 - 2.240
R-factor	0.2045
Rwork	0.204
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.561
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	128.800	2.360
High resolution limit [Å]	2.240	2.240
Rmerge	0.153	1.115
Rmeas	0.160	1.173
Rpim	0.047	0.361
Total number of observations	1003354	129703
Number of reflections	86227	12435
<I/σ(I)>	13.9	2.3
Completeness [%]	100.0
Redundancy	11.6	10.4
CC(1/2)	0.998	0.709

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Ammonium tartrate dibasic, PEG3350