9V5N
Crystal structure of anti HIV scFv against MPER region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.803, 183.919, 65.730 |
| Unit cell angles | 90.00, 93.60, 90.00 |
Refinement procedure
| Resolution | 37.650 - 2.540 |
| R-factor | 0.1965 |
| Rwork | 0.194 |
| R-free | 0.24480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.729 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.620 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Number of reflections | 33670 | 2706 |
| <I/σ(I)> | 11.72 | |
| Completeness [%] | 99.3 | 97.6 |
| Redundancy | 5.2 | 4.4 |
| CC(1/2) | 0.974 | 0.767 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293.15 | 0.5M SPG buffer pH4, 25% PEG 1500, 10% glycerol |
