9V5M
The crystal structure of RBBP4-H3 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.010, 77.463, 64.301 |
| Unit cell angles | 90.00, 90.61, 90.00 |
Refinement procedure
| Resolution | 38.730 - 2.100 |
| R-factor | 0.1692 |
| Rwork | 0.167 |
| R-free | 0.21090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.821 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.730 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.099 | 0.577 |
| Number of reflections | 27076 | 2716 |
| <I/σ(I)> | 10.8 | 3.1 |
| Completeness [%] | 98.1 | 98.87 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.995 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M Bis-Tris, pH 5.75, 28% PEG3350 |
