9V5D
Light driven CO2 reduction by fluorescent protein mRuby-CO2M2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-02 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.877, 95.530, 31.288 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.790 - 1.910 |
| R-factor | 0.22077 |
| Rwork | 0.218 |
| R-free | 0.28261 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.423 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.790 | 1.990 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 16871 | 2420 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 99.8 | |
| Redundancy | 13 | |
| CC(1/2) | 0.996 | 0.792 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M NH4HCO3, 0.2M (NH4)2SO4, 0.1M Bistris pH6.5, 24%PEG3350 |
