9V59
Crystal structure of calcium indicator WHaloCaMP1a labeled with BD566-HTL substrate
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-30 |
| Detector | STFC Large Pixel Detector |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.936, 94.184, 76.041 |
| Unit cell angles | 90.00, 101.34, 90.00 |
Refinement procedure
| Resolution | 32.795 - 2.175 |
| R-factor | 0.2075 |
| Rwork | 0.205 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549: ???) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.801 | |
| Rmeas | 0.992 | |
| Rpim | 0.141 | 0.576 |
| Number of reflections | 42186 | 2184 |
| <I/σ(I)> | 8.71 | 3.35 |
| Completeness [%] | 93.0 | 48.77 |
| Redundancy | 4.1 | 2.4 |
| CC(1/2) | 0.982 | 0.492 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | MES, Ammonium acetate, PEG3350 |
