9V56
Light driven bicarbonate reduction by fluorescent protein mRuby
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.370, 66.740, 94.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.630 - 1.940 |
| R-factor | 0.21668 |
| Rwork | 0.214 |
| R-free | 0.26487 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.470 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 1.990 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 1.493 | |
| Number of reflections | 15390 | 1118 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 99.9 | |
| Redundancy | 12.4 | |
| CC(1/2) | 0.997 | 0.617 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M NH4HCO3, 0.1M Bistris pH6.5, 25%PEG3350, |
