9V4Z
Crystal structure of Guanine-II riboswitch in complex with 8-oxoguanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.10213 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 37.820, 50.408, 219.758 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.940 - 2.740 |
| R-factor | 0.2111 |
| Rwork | 0.206 |
| R-free | 0.25650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.208 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (PHENIX 1.18.2-3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.880 | 2.890 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.149 | |
| Number of reflections | 5897 | 862 |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12 | |
| CC(1/2) | 0.999 | 0.904 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.08 M sodium chloride, 0.04 M sodium cacodylate trihydrate (pH 7.0), 30% v/v (+/-)-2-methyl-2,4-pentanediol, and 0.012 M spermine tetrahydrochloride |
