9V4G
Prenyltransferase Ord1 wild type
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-30 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.04500 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.000, 72.210, 84.190 |
| Unit cell angles | 86.04, 69.50, 90.02 |
Refinement procedure
| Resolution | 44.400 - 2.100 |
| R-factor | 0.2324 |
| Rwork | 0.229 |
| R-free | 0.24590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Initial phase from Se-SAD model |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.675 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2.4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.063 | 0.523 |
| Number of reflections | 74272 | 11833 |
| <I/σ(I)> | 10.58 | 2.25 |
| Completeness [%] | 97.6 | 96 |
| Redundancy | 3.2 | 3.4 |
| CC(1/2) | 0.998 | 0.774 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.1 M Bis-Tris,pH 5.5, 0.2 M Ammonium sulfate, 25% PEG 3350 |
