9V3P

Time-Resolved mixing crystallography structure of 1 micrometer Lysozyme microcrystals with 452 mM N-Acetyl-D-glucosamine at 2.5 seconds delay

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL2
Synchrotron site	SACLA
Beamline	BL2
Temperature [K]	293
Detector technology	CCD
Collection date	2024-04-21
Detector	MPCCD
Wavelength(s)	1.24
Spacegroup name	P 43 21 2
Unit cell lengths	79.500, 79.500, 38.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.550 - 1.810
R-factor	0.174
Rwork	0.172
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.926
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.680	1.840
High resolution limit [Å]	1.810	1.810
Number of reflections	11685	572
<I/σ(I)>	6.66
Completeness [%]	100.0
Redundancy	152.6
CC(1/2)	0.979	0.408

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	3	285	8% PEG 6000, 4.8 M NaCl, and 0.1M sodium acetate