9V3M

Time-Resolved mixing crystallography structure of 1 micrometer Lysozyme microcrystals with 226 mM N-Acetyl-D-glucosamine at 7.5 seconds delay

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL2
Synchrotron site	SACLA
Beamline	BL2
Temperature [K]	293
Detector technology	CCD
Collection date	2023-04-21
Detector	MPCCD
Wavelength(s)	1.24
Spacegroup name	P 43 21 2
Unit cell lengths	79.500, 79.500, 38.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.550 - 1.770
R-factor	0.183
Rwork	0.181
R-free	0.21910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.660
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.680	1.800
High resolution limit [Å]	1.770	1.770
Number of reflections	12477	620
<I/σ(I)>	6.85
Completeness [%]	100.0
Redundancy	172.5
CC(1/2)	0.980	0.429

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	3	285	8% PEG 6000, 4.8 M NaCl, and 0.1M sodium acetate