9V0O

Crystal structure of nanobody Tnb494 with MERS-CoV RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-30
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	83.885, 141.533, 153.295
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.650 - 2.700
Rwork	0.242
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.947
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.650	2.797
High resolution limit [Å]	2.700	2.700
Rmerge	0.064	2.017
Number of reflections	50801	4953
<I/σ(I)>	14.8
Completeness [%]	99.8
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	20% (w/v) PEG3000, citrate pH5.5