9V0L

UDP-binding PsBGluT, a tetrahydrobiopterin glucosyltransferase from Pseudanabaena sp. Chao 1811

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-05-06
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54056
Spacegroup name	P 61 2 2
Unit cell lengths	176.970, 176.970, 66.171
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	13.040 - 2.300
R-factor	0.2166
Rwork	0.214
R-free	0.26370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.682
Data reduction software	CrysalisPro (171.39.35c)
Data scaling software	Aimless (0.8.2)
Phasing software	MOLREP (11.9.02)
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.040	2.390
High resolution limit [Å]	2.300	2.300
Rpim	0.029	0.160
Number of reflections	27426	2848
<I/σ(I)>	24.7
Completeness [%]	99.4
Redundancy	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	285	0.1 M sodium acetate pH 5.0, 2M sodium formate