9UYV
Crystal Structure of VPS25 from Candidatus Prometheoarchaeum syntrophicum strain MK-D1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-16 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.722, 61.130, 88.200 |
| Unit cell angles | 90.00, 91.08, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| Rwork | 0.174 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.823 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.048 | 0.598 |
| Rmeas | 0.056 | 0.721 |
| Rpim | 0.029 | 0.398 |
| Number of reflections | 65977 | 3398 |
| <I/σ(I)> | 25.3 | 1.7 |
| Completeness [%] | 93.0 | |
| Redundancy | 3.6 | 3 |
| CC(1/2) | 0.723 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1M Tris, 20% PEG 6000, 0.2M Ammonium chloride |
