9UX0
Crystal structure of the virulence effector VepA in complex with its secretion chaperone VecA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-15 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 109.848, 152.934, 101.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.260 - 2.490 |
| R-factor | 0.2441 |
| Rwork | 0.241 |
| R-free | 0.27640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.676 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.260 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.147 | 1.108 |
| Rmeas | 0.160 | 1.193 |
| Rpim | 0.062 | 0.439 |
| Total number of observations | 195356 | 24194 |
| Number of reflections | 29792 | 3334 |
| <I/σ(I)> | 15 | 2 |
| Completeness [%] | 99.0 | |
| Redundancy | 6.6 | 7.3 |
| CC(1/2) | 0.995 | 0.608 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 100mM sodium cacodylate (pH 6.5), 20% (v/v) PEG 400, 200mM Li2SO4 |
