9UWO

Crystal structure of human galectin-10 produced by cell-free protein synthesis in complex with sucrose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	49.250, 49.250, 262.480
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.650 - 1.640
R-factor	0.2135
Rwork	0.211
R-free	0.24130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.117
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.740
High resolution limit [Å]	1.640	1.640
Number of reflections	24512	3819
<I/σ(I)>	23.1
Completeness [%]	99.9	99.7
Redundancy	70.1
CC(1/2)	0.991	0.639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293	cell-free crystallization