9UW1

Crystal structure of CDC48A-N domain in complex with PUX5 SHP box motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	60.070, 101.420, 110.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.750 - 1.880
R-factor	0.197
Rwork	0.196
R-free	0.22490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.688
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.753	1.988
High resolution limit [Å]	1.880	1.880
Number of reflections	48238	2412
<I/σ(I)>	13.2	1.4
Completeness [%]	94.9	57.9
Redundancy	13.6
CC(1/2)	0.999	0.582

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	0.2M Sodium malonate dibasic monohydrate 0.1M Bis-Tris Propane pH 8.5 20% w/v PEG 3350