9UVS

Crystal Structure of transaminase from Phaeobacter porticola

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	167.780, 60.410, 110.880
Unit cell angles	90.00, 106.25, 90.00

Refinement procedure

Resolution	29.860 - 1.730
R-factor	0.1682
Rwork	0.167
R-free	0.19440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.206
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.790
High resolution limit [Å]	1.730	1.730
Number of reflections	109205	5012
<I/σ(I)>	12.4
Completeness [%]	98.0
Redundancy	3.4
CC(1/2)	0.997	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2 M Magnesium chloride hexahydrate 0.1 M Imidazole pH 8.0 35% v/v (+/-)-2-Methyl-2,4-pentanediol