9UVM

The SeMet pili protein of Streptococcus Sanguinis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.900
Spacegroup name	P 21 21 21
Unit cell lengths	37.303, 58.874, 214.019
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.600 - 1.800
R-factor	0.2088
Rwork	0.208
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.656
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.410	1.840
High resolution limit [Å]	1.800	1.800
Number of reflections	45000	2634
<I/σ(I)>	9.9
Completeness [%]	100.0
Redundancy	7.3
CC(1/2)	0.997	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.3M Potassium sodium tartrate, 0.1M HEPES-HCL pH7.4.