9UVL

Crystal structure of AGAP1-GDP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2021-03-25
Detector	MAR CCD 300 mm
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	114.405, 124.678, 127.604
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.170 - 2.490
R-factor	0.221
Rwork	0.219
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	2.280
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.170	2.579
High resolution limit [Å]	2.490	2.490
Rmerge	0.086	1.570
Number of reflections	62085	5649
<I/σ(I)>	7.3
Completeness [%]	96.2
Redundancy	5.8
CC(1/2)	0.999	0.685

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		298	10% w/v PEG 4000, 20% w/v 2-Propanol, 100 mM HEPES Sodium Salt, pH 7.5