9UVH
Cystal Structure of Shark VNAR Nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-05-03 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.578, 34.486, 36.045 |
| Unit cell angles | 90.00, 109.95, 90.00 |
Refinement procedure
| Resolution | 19.950 - 1.800 |
| R-factor | 0.16539 |
| Rwork | 0.163 |
| R-free | 0.20255 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8hgi |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.940 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 7320 | 425 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 9.5 | |
| CC(1/2) | 0.999 | 0.976 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES pH 7.5, 20% w/v Polyethylene glycol 10,000 |
