9UVD

Crystal structure of Sec23a/24a/22b bound to STING pSGME motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-04
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.97915
Spacegroup name	C 1 2 1
Unit cell lengths	149.206, 96.020, 131.066
Unit cell angles	90.00, 90.26, 90.00

Refinement procedure

Resolution	64.710 - 2.980
R-factor	0.2178
Rwork	0.216
R-free	0.24950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.605
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.710	3.050
High resolution limit [Å]	2.980	2.980
Number of reflections	37851	2550
<I/σ(I)>	4.6
Completeness [%]	99.6
Redundancy	4.3
CC(1/2)	0.900	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50 mM Tris, pH 7.8, 0.3 M NaAc, 20% PEG 4000