9UUW
Crystal structure of Yarrowia lipolytica methionine gamma-lyase in complex with L-methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-17 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 166.054, 166.054, 81.314 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.782 - 3.040 |
| R-factor | 0.2344 |
| Rwork | 0.233 |
| R-free | 0.26620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.679 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.782 | 3.250 |
| High resolution limit [Å] | 3.040 | 3.040 |
| Rmerge | 0.136 | 0.241 |
| Rmeas | 0.192 | 0.341 |
| Rpim | 0.136 | 0.241 |
| Number of reflections | 25137 | 4518 |
| <I/σ(I)> | 6.6 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 1.9 | 1.9 |
| CC(1/2) | 0.954 | 0.582 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277.15 | 0.2M NaCl, 0.1M CAPS, pH 10.3, 20% PEG 8000 |
