9UUG
The structure of Egalitarian in complex with the K10 mRNA localization signal reveals a modular binding surface required for function
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 70.331, 70.331, 81.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.439 - 3.601 |
| R-factor | 0.2918 |
| Rwork | 0.291 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jyf |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.860 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.820 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmeas | 0.310 | 1.290 |
| Number of reflections | 4519 | 731 |
| <I/σ(I)> | 5.29 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.21 | |
| CC(1/2) | 0.992 | 0.928 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 50 mM Na Cocadylate pH 6.75, 1.2 M Li2SO4, 10 mM MgAc2 |
